Journal: The Journal of Experimental Medicine

Article Title: Interferon-α and Interleukin-12 Are Induced Differentially by Toll-like Receptor 7 Ligands in Human Blood Dendritic Cell Subsets

doi: 10.1084/jem.20020207

Figure Lengend Snippet: TLR-7 ligands induce the production of IL-12 and IFN-α from MDCs and PDCs, respectively. (A and B) After 24-h culture with various stimuli, concentration of IL-12 p40+p70 (A) and IFN-α (B) in the culture supernatants of MDCs, PDCs, and PBMCs were measured by ELISA. The data are shown by means ± SEM of five independent experiments. (C) After 5-h culture with R-848 (10 −6 M), intracellular staining of IL-12 and IFN-α in MDCs and PDCs, together with the staining of surface expression of CD40, was performed. Percentages of the respective cytokine producing DCs are indicated. This figure represents the results from one of three experiments.

Article Snippet: 10 μg/ml brefeldin A (Sigma-Aldrich) was added during the last 1 h. After the stimulation, MDCs and PDCs were stained with Cy-Chrome-labeled CD40 (5C3; BD PharMingen), and then fixed, permeabilized (FIX and PERM kit; Caltag Laboratories), and stained with FITC-labeled anti–IL-12 p40+p70 mAb (B-P24; DIACLONE Research) or unconjugated mouse anti–human IFN-α mAb (MC-16; Genzyme).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Staining, Expressing

Journal: Infection and Immunity

Article Title: Evaluation of Adenylate Cyclase Toxoid Antigen in Acellular Pertussis Vaccines by Using a Bordetella pertussis Challenge Model in Mice

doi: 10.1128/IAI.00857-17

Figure Lengend Snippet: Analysis of proinflammatory cytokines from lungs of mice immunized with aP titrations or aPs supplemented with RTX. (A to H) Th1-associated cytokines from supernatant of lung homogenates analyzed at days 1 and 3 postchallenge. Cytokines IL-6 (A and B), IL-17 (C and D), IFN-γ (E and F), and IL-1β (G and H) are shown. The blue boxes indicate aP groups, whereas aP-plus-RTX groups are indicated by gold triangles. Gray squares indicate mock-vaccinated and -challenged mice, while white squares represent nonvaccinated and nonchallenged mice (NC). Results are means ± SEM. ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.05. P values were determined by one-way ANOVA with Tukey's post hoc test compared to the PBS (naive) group of challenged mice. Brackets between two points represent unpaired two-way t test. A dashed line indicates the upper limit of detection, and a dotted line indicates the lower limit of detection.

Article Snippet: Quantitative analysis of the concentration of cytokines in the lungs of mice was determined with the Meso Scale Discovery V-PLEX proinflammatory panel (K15048G-1) or mouse IL-17 ultrasensitive kits (K152ATC-1) per the manufacturer's instructions.

Techniques: